Jurkat cells growing in DMEM supplemented with 10% FBS were collected and washed in ice-cold PBS. Cells were then lyzed in three vol ume of Kinase Extraction Buffer (70002-N80M in K435-40), keep on ice for 5 min, centrifuge at top speed for 15 min.

Item Number: BIOV7035-1
Unit of Measure: 1 MG
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    Manufacturer: BIOVISION
    Manufacturer Number: 7035-1
    Storage Temperature: Freezer (-80)

    Jurkat cells growing in DMEM supplemented with 10% FBS were collected and washed in ice-cold PBS. Cells were then lyzed in three vol ume of Kinase Extraction Buffer (70002-N80M in K435-40), keep on ice for 5 min, centrifuge at top speed for 15 min.