Jurkat cells growing in DMEM supplemented with 10% FBS were collected and washed in ice-cold PBS. Cells were then lyzed in three vol ume of Kinase Extraction Buffer (70002-N80M in K435-40), keep on ice for 5 min, centrifuge at top speed for 15 min.

Numero articolo: BIOV7035-1
Unità di misura: 1 MG
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    Produttore: BIOVISION
    Numero del produttore: 7035-1
    Temperatura di stoccaggio: Freezer (-80)

    Jurkat cells growing in DMEM supplemented with 10% FBS were collected and washed in ice-cold PBS. Cells were then lyzed in three vol ume of Kinase Extraction Buffer (70002-N80M in K435-40), keep on ice for 5 min, centrifuge at top speed for 15 min.